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If I double the length size of my chromatography column, what would be the resolution fold increment? Would it be 2X? Just as general question, I am not working with any column in particular.
07 August 2017 7,829 2 View
I am dialysing a 40KDa protein in a 3Kda dialysis membrane. The buffer that my protein is in has some glycerol remaining. So I want to make sure the glycerol is not there anymore. I ran some...
03 April 2016 5,525 5 View
I need to find intra-molecular interactions within a protein like hydrophobic, electrostatic, and van der Waals interactions. Do you know a program that can find this interactions and how to do...
01 February 2015 5,894 9 View
I am doing point mutations in PYMOL and I want to run molecular dynamics (MD) to see the effect of the mutation in the stability of my protein. In order to use the software Amber that does the MD...
31 December 2014 10,142 8 View
I work with a protein that is somewhat hydrophobic for which I use denaturing conditions to purify. After purification I refold it and dialyse it, but after the dialysis my protein precipitates a...
08 September 2013 685 11 View
Is it the triple negative one (TNBC)? I will be working with MDA-MB-231 breast cancer cells, a TNBC cells line.
04 May 2013 8,518 7 View
I would like to know the distance between the rings in a ring stacking interaction in proteins.
03 April 2013 6,321 4 View
I want to get a stock of my DNA of interest, but I only have transformed M15 cells and not DH5alpha cells. Is it ok if I do miniprep from an expression cell line like E. coli M15 culture and not...
11 December 2012 4,963 7 View
Does anyone know a commercial available and inexpensive protein that does not contain disulfide bonds?
10 November 2012 6,528 6 View
I successfully expressed my protein a while ago and suddenly it just stop expressing. I can't find out what the problem is, the DNA is inserted in a pQE30Xa plasmid and it has a very hydrophobic...
07 August 2012 6,801 13 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/
08 August 2024 4,585 1 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Exism movements after gaining power within liberal democracies under majority rule and independent rule of law system become permanent dictatorship threats, but why this is the case is not clear...
04 August 2024 8,125 3 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View