If I double the length size of my chromatography column, what would be the resolution fold increment?

07 August 2017 7,729 2 View

Can glycerol pass through a 3KDa dialysis membrane?

03 April 2016 5,428 5 View

How to find intramolecular interactions within a protein?

01 February 2015 5,825 9 View

How can I save a mutagenesis done in PYMOL as PDB file?

31 December 2014 9,958 8 View

Any suggestions on how to keep my protein soluble without using too much urea?

I work with a protein that is somewhat hydrophobic for which I use denaturing conditions to purify. After purification I refold it and dialyse it, but after the dialysis my protein precipitates a...

08 September 2013 632 11 View

What is the most aggressive type of breast cancer?

Is it the triple negative one (TNBC)? I will be working with MDA-MB-231 breast cancer cells, a TNBC cells line.

04 May 2013 8,441 7 View

What is the distance between the rings in a ring stacking interaction in proteins?

I would like to know the distance between the rings in a ring stacking interaction in proteins.

03 April 2013 6,159 4 View

Is it ok if I do miniprep of my DNA of interest from an expression cell line like E. coli M15 culture?

I want to get a stock of my DNA of interest, but I only have transformed M15 cells and not DH5alpha cells. Is it ok if I do miniprep from an expression cell line like E. coli M15 culture and not...

11 December 2012 4,907 7 View

Commercial available protein that does not contain disulfide bonds.

Does anyone know a commercial available and inexpensive protein that does not contain disulfide bonds?

10 November 2012 6,473 6 View

Protein no longer expressed

I successfully expressed my protein a while ago and suddenly it just stop expressing. I can't find out what the problem is, the DNA is inserted in a pQE30Xa plasmid and it has a very hydrophobic...

07 August 2012 6,744 13 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View