13 Questions 22 Answers 0 Followers
Questions related from Marisol Romero
If I double the length size of my chromatography column, what would be the resolution fold increment? Would it be 2X? Just as general question, I am not working with any column in particular.
08 August 2017 7,808 2 View
I am dialysing a 40KDa protein in a 3Kda dialysis membrane. The buffer that my protein is in has some glycerol remaining. So I want to make sure the glycerol is not there anymore. I ran some...
04 April 2016 5,494 5 View
Please Inbox for more information
08 August 2015 9,466 0 View
I need to find intra-molecular interactions within a protein like hydrophobic, electrostatic, and van der Waals interactions. Do you know a program that can find this interactions and how to do...
02 February 2015 5,871 9 View
I am doing point mutations in PYMOL and I want to run molecular dynamics (MD) to see the effect of the mutation in the stability of my protein. In order to use the software Amber that does the MD...
01 January 2015 10,059 8 View
I work with a protein that is somewhat hydrophobic for which I use denaturing conditions to purify. After purification I refold it and dialyse it, but after the dialysis my protein precipitates a...
09 September 2013 669 11 View
Is it the triple negative one (TNBC)? I will be working with MDA-MB-231 breast cancer cells, a TNBC cells line.
05 May 2013 8,496 7 View
I would like to know the distance between the rings in a ring stacking interaction in proteins.
04 April 2013 6,236 4 View
I want to get a stock of my DNA of interest, but I only have transformed M15 cells and not DH5alpha cells. Is it ok if I do miniprep from an expression cell line like E. coli M15 culture and not...
12 December 2012 4,945 7 View
Does anyone know a commercial available and inexpensive protein that does not contain disulfide bonds?
11 November 2012 6,509 6 View
I successfully expressed my protein a while ago and suddenly it just stop expressing. I can't find out what the problem is, the DNA is inserted in a pQE30Xa plasmid and it has a very hydrophobic...
08 August 2012 6,776 13 View
Does anyone know if there is a new program where I can predetermine the 3D structure of my protein just by giving the linear sequence?
07 July 2012 259 52 View
I expressed a cystein protease in M15 cells the vector is pQE30-xa. After lysis (8M urea in a tris buffer pH 8), the protein is in the pellet. I have tried many different things like 6M...
06 June 2012 8,449 13 View
