I don't have much cells. I centrifuged and did not get true pellet. Just pieces of white material. Now my cells were in about 6 ml medium. If I aspirate supernatant, they will have some cells. What to do in this case.
if you have few cells you may not be able to see a pellet. you could still decant the supernatant, leaving about 100 microlitres in the tube. Now add fresh media to this tube and transfer to a flask. Hopefully if the cells are not too few they should be able to grow. Sometimes if you plate extremely low numbers, cells may not grow well but that depends on the cell type you are using. It may be worth a try.
Hello Heba,
Your question is not clear. Please write details.
As far as I can understand, you want to derive cells from some biological fluid of patients and want to do FACS directly and you have low number of cells. Please tell, if the scenerio is same? What is the nature of fluid and how much volume you get and are you trying to stain with some antibody at the same time ?
Cheers!
First count both small pellets and super at any and check viability and count to know if the cells are really separated.
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