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Questions related from Ifeanyi Enekwa
Hi From experience does anyone know if there is a link between cell passage number and gene-editing efficiency? I suspect there might be an inverse relationship (ie high passage number = lower...
05 July 2021 4,737 2 View
Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...
30 January 2021 1,559 2 View
I would like to know if T7 in vitro transcription can be effectively terminated by pol II terminators (specifically bGH poly (A) signal)? And in reverse can a pol II promoter (eg CMV promoter)...
09 June 2020 9,568 0 View
Alternatively I would like to know if there's any bacterial promoters that are recommended for expressing small RNA molecules?
20 February 2020 1,206 4 View