Ifeanyi Enekwa

6 Questions 2 Answers 0 Followers

Questions related from Ifeanyi Enekwa

The exact termination site of nascent mRNA terminated by bGH polyA signal?

Hi Would anyone know where exactly a nascent eukaryotic mRNA transcript is terminated by the bGH polyA signal (referred to here as the first termination event). I know the pre-mRNA is usually...

11 October 2021 648 0 View

Cell passage number effect on gene-edting?

Hi From experience does anyone know if there is a link between cell passage number and gene-editing efficiency? I suspect there might be an inverse relationship (ie high passage number = lower...

05 July 2021 4,808 2 View

Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

30 January 2021 1,647 2 View

CRISPR-Cas9 targetting of gene promoters, any success?

Hi I have a set of three gRNAs targeting the promoter region of a specific gene of interest, the issue is that all three gRNAs work in vitro however are in effective in vivo in HEK293 cells and...

30 January 2021 8,825 2 View

I would like to know if T7 in vitro transcription can be effectively terminated by class II/ eukaryotic pol II terminators (specifically bGH)?

I would like to know if T7 in vitro transcription can be effectively terminated by pol II terminators (specifically bGH poly (A) signal)? And in reverse can a pol II promoter (eg CMV promoter)...

09 June 2020 9,632 0 View

If a guideRNA is cloned in a given plasmid, downstream of a U6 promoter would it be able to express in bacteria, specifically E coli in this case?

Alternatively I would like to know if there's any bacterial promoters that are recommended for expressing small RNA molecules?

20 February 2020 1,279 4 View