Why is the predicted molecular weight of the protein not authenticated by the SDS-PAGE result?

The molecular weight of my protein sequence is 41 kDa. The SDS-PAGE result demonstrated its size 36 kDa. What are the reasons behind that?

01 February 2018 7,130 3 View

Anyone could send me a book or manual related to site directed mutagenesis study?

I would lie to develop some mutants.

01 February 2018 3,785 0 View

What is promoter, motif and domain?

I would like to know the easy explanation for these tree points. I am happy if someone send me some articles or books about this.

01 February 2018 3,358 1 View

What is the possible reason for not getting the proper mRNA expression level in qRT-PCR compared to the western blot protein data?

I got the excellent protein quantity after measuring the protein level through western blot analysis. Hence I am checking the corresponding mRNA in the qRT-PCR but failed to get proper mRNA...

10 November 2017 3,246 14 View

Please tell me any link address for user friendly free gene network analysis software?

Gene , Molecular Biologist, Biostatics

10 November 2017 8,246 0 View

Anyone could tell me the actual state of Na2S in water?

I would like to know the sodium sulfide actual state level in the solution. Biochemistry, Chemistry, Polymer chemistry, Biophysics

05 June 2017 4,669 7 View

Any one could send me paper related Intra fragment interaction energy calculation between substrate and enzyme?

Molecular Biology, Molecular docking, Biophysics

03 April 2017 8,043 0 View

What should be the possible reasons that hinders substrate (ligand) to bind in the active site of receptor (protein)?

Enzymology, Molecular biology, Biophysics

02 March 2017 7,870 3 View

I would like to calculate the competitive and non competitive inhibition of enzyme. Could anyone send me a easy method for that?

11 December 2016 4,010 0 View

Is the cofactor considered as the vital force for the enzyme synthesis?

We are performing enzymatic reaction adding the cofactor for that enzyme. Without adding the cofactor the assay condition is suitable.

11 December 2016 1,682 4 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

23 January 2021 2,096 4 View