If 0.9 mg/mL of an enzyme catalyzes the conversion of 25 micromoles of substrate per unit, what will be the unit of enzyme activity?

I would like to know the answer and the solution procedure of this problem.

31 December 2019 5,348 3 View

Why is the predicted molecular weight of the protein not authenticated by the SDS-PAGE result?

The molecular weight of my protein sequence is 41 kDa. The SDS-PAGE result demonstrated its size 36 kDa. What are the reasons behind that?

01 February 2018 7,130 3 View

Anyone could send me a book or manual related to site directed mutagenesis study?

I would lie to develop some mutants.

01 February 2018 3,785 0 View

What is promoter, motif and domain?

I would like to know the easy explanation for these tree points. I am happy if someone send me some articles or books about this.

01 February 2018 3,358 1 View

What is the possible reason for not getting the proper mRNA expression level in qRT-PCR compared to the western blot protein data?

I got the excellent protein quantity after measuring the protein level through western blot analysis. Hence I am checking the corresponding mRNA in the qRT-PCR but failed to get proper mRNA...

10 November 2017 3,246 14 View

Please tell me any link address for user friendly free gene network analysis software?

Gene , Molecular Biologist, Biostatics

10 November 2017 8,246 0 View

Anyone could tell me the actual state of Na2S in water?

I would like to know the sodium sulfide actual state level in the solution. Biochemistry, Chemistry, Polymer chemistry, Biophysics

05 June 2017 4,669 7 View

What should be the possible reasons that hinders substrate (ligand) to bind in the active site of receptor (protein)?

Enzymology, Molecular biology, Biophysics

02 March 2017 7,870 3 View

I would like to calculate the competitive and non competitive inhibition of enzyme. Could anyone send me a easy method for that?

11 December 2016 4,010 0 View

Is the cofactor considered as the vital force for the enzyme synthesis?

We are performing enzymatic reaction adding the cofactor for that enzyme. Without adding the cofactor the assay condition is suitable.

11 December 2016 1,682 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View