Md. Harun-Ur- Rashid

20 Questions 21 Answers 0 Followers

Questions related from Md. Harun-Ur- Rashid

If 0.9 mg/mL of an enzyme catalyzes the conversion of 25 micromoles of substrate per unit, what will be the unit of enzyme activity?

I would like to know the answer and the solution procedure of this problem.

01 January 2020 5,522 3 View

What is promoter, motif and domain?

I would like to know the easy explanation for these tree points. I am happy if someone send me some articles or books about this.

02 February 2018 3,520 1 View

Anyone could send me a book or manual related to site directed mutagenesis study?

I would lie to develop some mutants.

02 February 2018 3,986 0 View

Why is the predicted molecular weight of the protein not authenticated by the SDS-PAGE result?

The molecular weight of my protein sequence is 41 kDa. The SDS-PAGE result demonstrated its size 36 kDa. What are the reasons behind that?

02 February 2018 7,355 3 View

Please tell me any link address for user friendly free gene network analysis software?

Gene , Molecular Biologist, Biostatics

11 November 2017 8,426 0 View

What is the possible reason for not getting the proper mRNA expression level in qRT-PCR compared to the western blot protein data?

I got the excellent protein quantity after measuring the protein level through western blot analysis. Hence I am checking the corresponding mRNA in the qRT-PCR but failed to get proper mRNA...

11 November 2017 3,487 14 View

Anyone could tell me the actual state of Na2S in water?

I would like to know the sodium sulfide actual state level in the solution. Biochemistry, Chemistry, Polymer chemistry, Biophysics

06 June 2017 4,848 7 View

Any one could send me paper related Intra fragment interaction energy calculation between substrate and enzyme?

Molecular Biology, Molecular docking, Biophysics

04 April 2017 8,206 0 View

What should be the possible reasons that hinders substrate (ligand) to bind in the active site of receptor (protein)?

Enzymology, Molecular biology, Biophysics

03 March 2017 8,098 3 View

Is the cofactor considered as the vital force for the enzyme synthesis?

We are performing enzymatic reaction adding the cofactor for that enzyme. Without adding the cofactor the assay condition is suitable.

12 December 2016 1,841 4 View

I would like to calculate the competitive and non competitive inhibition of enzyme. Could anyone send me a easy method for that?

Enzyme kinetics analysis, Biochemistry, Molecular Biology, Biophysics

12 December 2016 4,178 0 View

What is the possible reason behind that the enzyme lose its activity after removing GST-TAG?

Before removing the GST-Tag my enzyme produce cysteine but after removing the tag it is not showing its activity. Plant Molecular Biology, Proteomics

10 October 2016 3,730 6 View

Could anyone send me the genetotypic and phenotypic correlation and diversity analysis softwear?

Plant Breeding, Genetics, Statistics

10 October 2016 2,789 3 View

Anyone could give me the synthesize protocol for 3-hydroxy-4- pyridone?

Biochemistry, Chemistry

08 August 2016 9,249 0 View

Could anyone give me the sequence of house keeping genes of M. pudica for quantitative real time PCR experiment?

Molecular Biology and molecular genetics

08 August 2016 4,955 8 View

Is it correct to use the predicted protein sequence data from NCBI for phylogenetic tree making?

Molecular Biology, Molecular Genetics, Bioinformatics

08 August 2016 6,827 1 View

Could anyone send me the protocol for mimosine synthase assay?

The searchers who are dealing with recombinant technology

06 June 2016 9,795 2 View

Any one could give me an easy method for Agrobacterium mediated genetic transformation?

I would like to make GM crops of M. pudica and L. leucocephala.

03 March 2016 892 3 View

Any one could send me an easy protocol for M. pudica and L. leucocephala plant regeration in tissue culture?

Leaf and bud culture is good for me

03 March 2016 2,910 3 View

I am trying to make SDS-PAGE gel. But unfortunately the running gels are not hardening. What is the reason behind that?

I have added the following composition- 1600 uL 30%acrylamide2000 uL pH8.8 3M tris HCl, 40 uL 10% SDS 320 uL DW 33.3 uL 25%APS3.2uL TEMED

01 January 2016 895 51 View