If 0.9 mg/mL of an enzyme catalyzes the conversion of 25 micromoles of substrate per unit, what will be the unit of enzyme activity?

I would like to know the answer and the solution procedure of this problem.

31 December 2019 5,348 3 View

Why is the predicted molecular weight of the protein not authenticated by the SDS-PAGE result?

The molecular weight of my protein sequence is 41 kDa. The SDS-PAGE result demonstrated its size 36 kDa. What are the reasons behind that?

01 February 2018 7,130 3 View

Anyone could send me a book or manual related to site directed mutagenesis study?

I would lie to develop some mutants.

01 February 2018 3,785 0 View

What is promoter, motif and domain?

I would like to know the easy explanation for these tree points. I am happy if someone send me some articles or books about this.

01 February 2018 3,358 1 View

What is the possible reason for not getting the proper mRNA expression level in qRT-PCR compared to the western blot protein data?

I got the excellent protein quantity after measuring the protein level through western blot analysis. Hence I am checking the corresponding mRNA in the qRT-PCR but failed to get proper mRNA...

10 November 2017 3,246 14 View

Please tell me any link address for user friendly free gene network analysis software?

Gene , Molecular Biologist, Biostatics

10 November 2017 8,246 0 View

Anyone could tell me the actual state of Na2S in water?

I would like to know the sodium sulfide actual state level in the solution. Biochemistry, Chemistry, Polymer chemistry, Biophysics

05 June 2017 4,669 7 View

Any one could send me paper related Intra fragment interaction energy calculation between substrate and enzyme?

Molecular Biology, Molecular docking, Biophysics

03 April 2017 8,043 0 View

What should be the possible reasons that hinders substrate (ligand) to bind in the active site of receptor (protein)?

Enzymology, Molecular biology, Biophysics

02 March 2017 7,870 3 View

I would like to calculate the competitive and non competitive inhibition of enzyme. Could anyone send me a easy method for that?

11 December 2016 4,010 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View