Can we convert the graphene oxide films into rGO films without actually removing graphene oxide from the substrate?

What are the possible ways to convert GO to rGO?

19 October 2020 2,875 4 View

How to prepare MoS2 nanoparticles and 2D material using chemical method?

How to prepare MoS2 nanoparticles and 2D material using chemical method? Is MoS2 a transparent material, what about the absorbance of the MoS2?

12 October 2020 1,874 3 View

Can any one tell me is there any relation between the density rate and the grain size?

I am working on the ferroelectric ceramics and i am studying the sintering effect on the ceramics. Does the rate of density has any connection to the grain size of the ceramics. How does the grain...

22 April 2019 534 12 View

I am doing inmpedance measurements for batio3 based ceramics between the temperature 25-150 degrees celcius.Why I am getting only straight lines?

At what temperatures we get semicircles for impedance measurements?I have tried up to 150 degrees Celsius for BaTiO3 based ceramics but i could get only straight line.Do i need to go for higher...

05 March 2019 9,219 4 View

How can I approximate the Poynting Vector in a slot waveguide for a TE mode, in COMSOL?

23 July 2015 5,360 1 View

Hints or tricks to design a Slot waveguide photonic modulator with COMSOL as a COMSOL beginner ?

After reading some papers on photonic organic modulators, I started evaluation of COMSOL to optimize the overlap of the RF electrical field and the optical field of the travelling wave.The layout...

07 January 2015 4,911 8 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

31 December 2020 7,433 5 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View