Antibody Specification for Immune precipitation detection.

During Immune precipitation is that possible to use same antibody race, which are use for western bloting for detection. E.g Suppose my protein X I.P which race is Rabbit and detected this protein...

01 February 2016 9,446 4 View

"The SEM is a population statistics, not a sample statistic". may I used SEM for relatively smaller population statistics ?

10 November 2014 7,458 2 View

Can anyone tell me if U937 cells lines are derived from leukomic or lymphatic cells?

Can any body let me know which one is right?

07 August 2013 3,140 2 View

How to relate cancer and inflammation in vitro and in vivo cancer models?

In cancer related inflammation which parameter do I have to check first for in vitro and in vivo study?

02 March 2013 281 2 View

How to assess cell proliferation assays using BrdU by flow cytometry?

Can I use BrdU antibody for this flow study. How to to gate them?

02 March 2013 5,182 1 View

Can anyone offer advice regarding liver tissue lysate preparation for western blot analysis?

How to make tissue lysate samples?

10 November 2012 4,987 10 View

After inducing systemic inflammation (Endotoximea) in mice in duration of 7 days, is there any change in body weight as compared to normal controls?

When the mortality is about 50%, is any significance related to their mortality due to body weight?

05 June 2012 786 7 View

Any body help me regarding isolation of bronchiolar alveolar lavage from rat or mice?

I want to collect bronchiolar alveolar lavage from rat or mice...and see the cell count their

01 February 2012 3,012 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View