Any greater explanation?? and is the EC50 oe IC50 calculation similar for cell line effect?
Can you explain what produced this output for the IC50?
During Immune precipitation is that possible to use same antibody race, which are use for western bloting for detection. E.g Suppose my protein X I.P which race is Rabbit and detected this protein...
01 February 2016 9,528 4 View
Biostatistic .
10 November 2014 7,530 2 View
Can any body let me know which one is right?
07 August 2013 3,210 2 View
In cancer related inflammation which parameter do I have to check first for in vitro and in vivo study?
02 March 2013 372 2 View
Can I use BrdU antibody for this flow study. How to to gate them?
02 March 2013 5,269 1 View
How to make tissue lysate samples?
10 November 2012 5,060 10 View
When the mortality is about 50%, is any significance related to their mortality due to body weight?
05 June 2012 859 7 View
I want to collect bronchiolar alveolar lavage from rat or mice...and see the cell count their
01 February 2012 3,082 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.
06 August 2024 8,848 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View