IC50 is 0.4584 to 2.234e+012 ??? What does it mean??

Any greater explanation?? and is the EC50 oe IC50 calculation similar for cell line effect?

05 June 2016 4,793 1 View

Antibody Specification for Immune precipitation detection.

During Immune precipitation is that possible to use same antibody race, which are use for western bloting for detection. E.g Suppose my protein X I.P which race is Rabbit and detected this protein...

01 February 2016 9,446 4 View

"The SEM is a population statistics, not a sample statistic". may I used SEM for relatively smaller population statistics ?

10 November 2014 7,458 2 View

Can anyone tell me if U937 cells lines are derived from leukomic or lymphatic cells?

Can any body let me know which one is right?

07 August 2013 3,140 2 View

How to assess cell proliferation assays using BrdU by flow cytometry?

Can I use BrdU antibody for this flow study. How to to gate them?

02 March 2013 5,182 1 View

Can anyone offer advice regarding liver tissue lysate preparation for western blot analysis?

How to make tissue lysate samples?

10 November 2012 4,987 10 View

After inducing systemic inflammation (Endotoximea) in mice in duration of 7 days, is there any change in body weight as compared to normal controls?

When the mortality is about 50%, is any significance related to their mortality due to body weight?

05 June 2012 786 7 View

Any body help me regarding isolation of bronchiolar alveolar lavage from rat or mice?

I want to collect bronchiolar alveolar lavage from rat or mice...and see the cell count their

01 February 2012 3,012 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View