Western blot with very acidic proteins, any suggestions?

I am trying to do a Western Blot to detect acidic proteins (~30kDa) that have a PI of ~3.5, due to the presence of Asp and Glu repeats. The proteins are about 80% pure. After I ran them on...

24 January 2021 7,226 3 View

Problems with allele speration by cloning in pKS plasmid?

I am trying to do the allelic separation of a ~8 kb region using cloning strategy with pKS plasmid. However, when I analyze the result of the colony PCR sequencing It seems that both alleles were...

14 December 2020 5,829 2 View

At what concentration does hydrogen peroxide inhibit enzyme activity?

I am trying to measure GUS expression, and was wondering if anyone knew what concentration hydrogen peroxide inhibits the GUS protein??? Thanks ! Any help is appreciated!

23 November 2020 4,101 3 View

Does anyone know a Python script bank to analyze data from thermal images / thermographs?

script, phyton and thermography

13 September 2020 3,951 3 View

Management Innovation and Bahavior economic?

Hello guys! I'm looking for studies that address behavioral economics in Management innovation. Especially regarding the decision-making process.

13 September 2020 8,687 3 View

How to quantify and stratify the volume and specific surface area of submicro-, micro-, meso- and macropores in a porous solid?

Is it possible to quantify and stratify the kinds of pores (volume and specific surface area)? Only N2 physisorption is enough? Is there another technique to help this? Physically are there other...

04 August 2020 9,175 3 View

Is possible connect infrared camera - Flir One Pro 3td generation in windows or linux, to capture images and videos?

I found in internet some drivers and softwares (flironecontrol and others), If anyone knows about more softwares, please share.

29 June 2020 6,641 2 View

What else reduces NBT (nitroblue tetrazolium) to formazan?

I need to produce formazan to make a standard curve, (as in this paper:Quantitative Determination of Superoxide in Plant Leaves Usi... ) The reaction requires riboflavin and methionine,...

05 March 2020 6,403 3 View

Could research community help me to sort out the problem related with structural equation model(SEM)?

I am trying to examine the impact of cognitive and psychological factors, socio-demographic factors, Experimental processing factors and socio-cultural and institutional factors on farmers risk...

14 May 2019 4,341 1 View

Dear colleagues, please lmn what volume do you use for intranasal instillation in rats?

I am in doubt as to the best volume for intranasal instillation in rats, what volume ensures the best distribution of the particles?

20 February 2019 2,920 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View