I would like to know if in PCA medium i can look fungi growth?

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13 February 2021 9,183 9 View

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The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

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What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

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Difficulty in cloning of 1kb gene into vector?

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Can part of recombinant protein not be expressed?

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Giorgia Pertile

Try PDA (Potato Dextrose Agar).

https://microbiologyinfo.com/potato-dextrose-agar-pda-principle-uses-composition-procedure-and-colony-characteristics/

Nina Shishkoff

You probably can, but more information would be helpful: are you trying to count a particular type of fungus, or total colonies of all fungi? Do you need to be quantitative? Are you isolating them out of soil or what? Is there a problem with bacterial contamination? Are you also interested in Oomycetes? Are you interested only in fast-growing fungi, or are you also interested in slow-growers who might not be detectable on the same medium? Have other scientists tried to do what you are trying to do and described their methods? Every method of counting has its artifacts, so you want to pick a method where the artifacts aren't too severe.

Malik Nawaz Shuja

if possible kindly share some more detail of your work and picture of fungi.