We want to determine the number of primary hepatocytes, live/dead, that we retrieve from cell isolation.
Jarosite is obtained from bioleaching residue and I want to determine if there are increased active sites biogenic jarosite compared to chemical synthetic jarosite.
10 July 2024 8,132 2 View
Scales?
10 July 2024 1,176 2 View
Hi, I would like to gain an insight on the various methods of measuring biomass and growth of Aspergillus oryzae, which you have personally tried and would gladly recommend. Thanks.
08 July 2024 6,057 2 View
Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...
27 June 2024 5,068 1 View
The major causes of post harvest loss of produce in Ghana.
20 June 2024 6,445 5 View
Hi, I want to analyze the protein expression levels of HPV16 E6/E7 in the cervical cancer cell lines SiHa and CaSki after treatment with silencing RNA for this oncogene. Can anyone recommend a...
03 June 2024 9,579 3 View
I'm implanting Alzet pumps in mice with a brain infusion cannula to deliver into the ICV space. I am familiar with the technique that includes removing the pump from the tubing and then using a...
22 May 2024 7,121 3 View
Hi, I am performing optimization calculations in VASP for oxides. After my calculation was finished, I checked the OUTCAR file, and the optimization finished well. But when I checked the other...
29 April 2024 1,506 2 View
Hello, I have data in SPSS from a scale I did, I have 1 variable which is age (separated into 3 categories using values of 1-3), a 2nd variable which is gender (again separated into 3 categories...
19 April 2024 9,983 3 View
We are trying to use the Life Diagnostics Trout HSP70-23 to look at Heat Shock Protein 70 in fish red blood cells to look at physiological changes in response to stressors. I have found very...
08 April 2024 1,134 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View