my cells were ofen contaminated,I suspect there exists the mycoplasma. I want to detect the mycoplasma by realtime PCR, would you give me a available primer sequence?
Dear Wang Nana
Pls. find the attached files, may help in your research, good luck
https://www.researchgate.net/profile/Martin_Altwegg/publication/12461439_Rapid_detection_of_Mycoplasma_pneumoniae_in_clinical_samples_by_real-time_PCR/links/09e4150e800946e219000000.pdf
http://www.criver.com/files/pdfs/bps/mycoseq_mycoplasma_detection_test.aspx
http://www.ncbi.nlm.nih.gov/pubmed/23104288
http://www.jove.com/video/3057/optimized-pcr-based-detection-of-mycoplasma
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200028
https://www.abmgood.com/PCR-Mycoplasma-Detection-Kit-G238.html
http://jcm.asm.org/content/46/9/3116.full
http://www.sigmaaldrich.com/catalog/product/sigma/mp0035?lang=en®ion=SY
https://www.researchgate.net/profile/Jorgen_Jensen2/publication/8882633_Use_of_TaqMan_5'_nuclease_real-time_PCR_for_quantitative_detection_of_Mycoplasma_genitalium_DNA_in_males_with_and_without_urethritis_who_were_attendees_at_a_sexually_transmitted_disease_clinic/links/00b7d526ed11df2045000000.pdf
http://jcm.asm.org/content/40/4/1451.short
Regards
Prof. Houda Kawas
Article Rapid detection of Mycoplasma pneumoniae in clinical samples...
Article Use of TaqMan 5′ Nuclease Real-Time PCR for Quantitative Det...
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