I would like to configure the spectrophotometer to 630 nm for a siderophore assay. Do you have any information about operating this machine?

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What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Why do different methods of accessing BindingDB yield different predicted targets for the same SMILES?

How can we determine the reliability of the predicted targets of BindingDB, when different methods of accessing them yield different results for the same SMILES? For example, using the ‘Find My...

06 January 2024 3,551 0 View

What is the appropriate wavelength for measuring bacteria?

especially for Bacillus

25 October 2023 1,926 0 View

Can You Overcoming Solubility Issues in Assessing Cell Toxicity Effects of n-Hexane Herbal Fraction?

Colleagues, I am currently working on a project studying the toxicological effect of an n-Hexane fraction of a herbal extract using a clonogenic assay. However, I'm encountering difficulties as...

30 May 2023 8,946 5 View

Some parts of DD Solver Program are'nt active and dont work. Would you please help me?

Peace be upon you I have downloaded DD Solver software as an add-in on Excel. However, some parts of it do not seem to work. please guide me. Sincerely Yours: Fariborz Kaveh

09 November 2021 6,115 1 View

how can sponge iron be grinded to micronized iron powder without oxidation during ball milling? which method is the best for sponge iron grinding?

Our aim is to produce Powder metallurgy grade micronized iron powder by using sponge iron. The problem is that at last stage of powder production when powder is crossing hydrogen belt furnace, it...

20 August 2021 8,079 5 View

Is there a MATLAB code for Zernike coefficients calculation from xy wavefront error data ?

I have the wavefront aberration error (or OPD) data in xy plane. Know I want use MATLAB to extract the Zernike coefficients from the data set. it would be great if any one could help me to do it....

22 January 2021 4,654 3 View

Hello to every one. I have a color image and want to remove the lines and numbers from it with MATLAB. but how ?

I want a smooth image without these lines and numbers but i don't know how

27 December 2020 1,955 10 View

How write matlab code for multipath rayleigh fading channel?

in my case we assume independent multi path rayleigh fading channels, and the power gains of multiple paths are exponentially distributed. the numbers of channel paths is 4 and the TX-RX...

10 February 2019 4,891 2 View

GRADE approach for meta-analysis on diagnostic test accuracy studies?

Hello all We have conducted a meta-analysis on diagnostic test accuracy studies that their method was similar to comparison method studies (We had data on index test, Reference test and the...

08 November 2018 1,235 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Is there a method to measure the concentration of Cs, Sr, Co using spectrophotometer?

it's important to know that we are using the inactive elements of Cs, Sr, Co

25 July 2024 1,553 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View