especially for Bacillus
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
How can we determine the reliability of the predicted targets of BindingDB, when different methods of accessing them yield different results for the same SMILES? For example, using the ‘Find My...
06 January 2024 3,551 0 View
Hello, I would like to configure the spectrophotometer to 630 nm for a siderophore assay. The spectrophotometer we will be using is the Beckman Coulter DU730. Do you have any information about...
25 October 2023 7,915 2 View
Colleagues, I am currently working on a project studying the toxicological effect of an n-Hexane fraction of a herbal extract using a clonogenic assay. However, I'm encountering difficulties as...
30 May 2023 8,946 5 View
Peace be upon you I have downloaded DD Solver software as an add-in on Excel. However, some parts of it do not seem to work. please guide me. Sincerely Yours: Fariborz Kaveh
09 November 2021 6,115 1 View
Our aim is to produce Powder metallurgy grade micronized iron powder by using sponge iron. The problem is that at last stage of powder production when powder is crossing hydrogen belt furnace, it...
20 August 2021 8,079 5 View
I have the wavefront aberration error (or OPD) data in xy plane. Know I want use MATLAB to extract the Zernike coefficients from the data set. it would be great if any one could help me to do it....
22 January 2021 4,654 3 View
I want a smooth image without these lines and numbers but i don't know how
27 December 2020 1,955 10 View
in my case we assume independent multi path rayleigh fading channels, and the power gains of multiple paths are exponentially distributed. the numbers of channel paths is 4 and the TX-RX...
10 February 2019 4,891 2 View
Hello all We have conducted a meta-analysis on diagnostic test accuracy studies that their method was similar to comparison method studies (We had data on index test, Reference test and the...
08 November 2018 1,235 2 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
11 July 2024 7,847 1 View
I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...
27 June 2024 2,951 5 View
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15 June 2024 6,072 2 View
This jelly gets precipitated during ammonium sulfate precipitation
06 June 2024 5,631 4 View
Hi, i am doing research on the potential antimicrobial activity of Bacillus against s.aureus.i have tried various methods for six months.. like soft agar overlay method but my bacillus colony keep...
26 May 2024 2,151 6 View
I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...
02 May 2024 4,928 0 View
As a new microbiologist, I am currently working in a non-sterile production pharmaceutical factory where I take pure water samples from 13 points, fill 100 ml of pure water into 3 sample...
29 April 2024 7,096 1 View
I have some environmental isolates that we are looking to identify. Morphologically, the majority of the isolates are Bacillus spp. We would like to use the API 50 CHB/E system and would like to...
28 April 2024 9,559 0 View
Please provide an answer along with the reference.
26 March 2024 7,488 2 View
I and using vectors with autoinducibe promoters(Pylb and cyt1). I have performed SDS of 24, 48, 72, 96 and 120 hours. No expression is found in soluble or insoluble form.
14 March 2024 8,752 0 View
