Hi Edith,

For a successful Epithelial to Mesenchymal Transition you can use a relatively new and simple method offered by R&D Systems. All you need to do is to add EMT inducing media supplement to your cells and incubate them for few days.

For more details follow the links below:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856330/

https://www.rndsystems.com/products/stemxvivo-emt-inducing-media-supplement-100x-_ccm017

Radhwan Al-Zidan thank you for this suggestion. I’ll certainly take a look! Are you familiar with inducing EMT in transfected cells (any cell line) by adding only TGF beta 1 to media as the inducing supplement?

Moreover, you can stimulate EMT e.g. via TGF-beta

Thank you Andreas Eisenreich!