For a successful Epithelial to Mesenchymal Transition you can use a relatively new and simple method offered by R&D Systems. All you need to do is to add EMT inducing media supplement to your cells and incubate them for few days.
Radhwan Al-Zidan thank you for this suggestion. I’ll certainly take a look! Are you familiar with inducing EMT in transfected cells (any cell line) by adding only TGF beta 1 to media as the inducing supplement?