Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

Can someone explain blocking vs. nonblocking electrodes in terms of electrochemical impedance spectroscopy?

I have fabricated thin films and want to measure the impedance parallel to the material. I suspect the films are conducting protons and electrons based on some Hall effect measurements I have...

27 January 2021 1,712 5 View

Isoabsorbance and 3D Plots are missing under the "Spectra" toolbar of Agilent Chemstation 1200 series software. How can I retrieve it please?

The Problem started after replacing the HPLC Pump from Quaternary to Isocratic pump due to pump failure. The configuration was set as the previous set up as Modular 3D LC System and the license...

11 October 2020 8,018 3 View

I am using a rat TNFa ELISA to measure TNFa in culturing media. How soon following my alcohol treatment should I collect and measure TNFa?

I am using an organotypic hippocampal slice culture where we are exposing our slices to alcohol with subsequent alcohol withdrawal. I want to make sure I am capturing the right time to assess...

16 September 2020 7,020 2 View

Has anyone using organotypic hippocampal slice culture models observed holes in their tissue specimens between 6-12 days in vitro?

Our lab has noticed that after several tissue preparations for organotypic hippocampal slice cultures, some of our tissue is developing what appears to be large holes between the CA1 and dentate...

03 March 2020 3,981 3 View

How do I graph RT-PCR amplification values?

I have conducted an experiment to investigate the up-regulation and down-regulation of immune related genes. I have calculated the PCR efficiency and used the pfaffl method to get amplification...

22 October 2019 1,231 1 View

Is Learning Analytics under Data Mining?

In what ways EDM and LA differ and does LA under Data Mining and does it use the same techniques from Data Mining?

30 April 2019 8,370 1 View

LPS effect on TNF production. Time course over 7 days?

Does anybody know what happens to TNF alpha in PBMC after LPS stimulation? Or in MLR assay after LPS stimulation? Over more than 24 h i.e. up to 7 days?

14 February 2019 2,659 3 View

Does anyone have cell culture experience w/ human mammary epithelial cells &/or has tried to induce EMT in any cell line including epithelial cells?

Does anyone have cell culture experience w/ human mammary epithelial cells &/or has tried to induce EMT in any cell line including epithelial cells? Also any suggestions on doing scratch...

20 January 2019 5,860 6 View

Does anyone have cell culture experience w/ human mammary epithelial cells &/or has tried to induce EMT in any cell line including epithelial cells?

Does anyone have cell culture experience w/ human mammary epithelial cells &/or has tried to induce EMT in any cell line including epithelial cells? Also any suggestions on doing scratch...

31 December 2018 2,045 7 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View