I wonder what progress has already been made on transfer of nitrogenase from bacteria and cyanobacteria to higher plants?

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Adam B Shapiro

Here is a recent paper on the subject:

"Expression of Active Subunit of Nitrogenase via Integration into Plant Organelle Genome"

https://www.ncbi.nlm.nih.gov/pubmed/27529475

Anita Sharma

The basic problem in cloning prokaryotic nif gene in Eukaryotas is the requirement of expression. if expression takes place then functioning will affect plant growth as nif activity requires high amount of energy as ATP, which otherwise inhibit plant growth and other properties.

Marius K. Somda

Dear

I recommend you to use CRIS/PR technology to transfert gene from prokaryotic cell to eukaryotic cell.

Thank you

Marius