Any progress on reducing the ability of the enzyme Rubisco to use oxygen as substrate?

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What are the reasons behind the affinity of the enzyme Rubisco for both oxygen and carbon dioxide?

we know that the enzyme Rubisco use both oxygen and carbon dioxide as substrates,but the reasons behind such affinity for both substrates is not clear.

11 December 2016 2,489 1 View

How successful are the attempts to produce C4 plants from C3 plants??

three photosynthetic pathways are known to exist in in higher land plants,C3 ,C4, and C A M.I wonder, if attempts to produce C4 plants from C3 plants are successful?

11 December 2016 3,159 5 View

I wonder what progress has already been made on transfer of nitrogenase from bacteria and cyanobacteria to higher plants?

we are working on the molecular basis of salinity tolerance in plants.accordingly, we are interested in the transfer of nitrogenase enzymes to higher plants.

10 November 2016 6,470 3 View

I wonder ,if blue roses are available on the market via plant genetic engineering?

we are very much interested in plant biotechnology,in particular the moleculare basis of salinity tolerance in plants.we are also interested in anti-sense RNA technology to alter the color of...

01 January 1970 5,365 8 View

How effective is the anti-sense RNA technology in manipulating sodium uptake by plant roots?

we know t that anti sense RNA technology is very effective in certain cases, such as the production of Flavr Savr tomato. how effective is this technology in reducing sodium uptake by plant...

01 January 1970 3,230 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View