How to decide on annealing temperature for primer pairs in RT-qPCR?

Hello i am trying a pair of new primers. Unfortunately I do not have enough reactions to perform a temperature gradient. My HK primer has Tm of 60.3 (forward) and 60.8 (reverse) and the other...

05 March 2019 186 7 View

Maslach Burnout Inventory Student Form, reliability of professional efficacy?

Dear all, We conducted a research on college students using Maslach Burnout Inventory-Student Form of Schaufeli et. 2002. As you all know this scale consists of three factors, namely exhaustion,...

22 February 2019 7,488 1 View

Is there a min amount of cells per samples in ROMO1 ELISA?

Hello everyone, I am pretty new to ELISA and I have been thinking of conducting a ROMO1 quantitative sandwich ELISA with NHDFa. I am planning on having three samples in triplicates however in our...

21 November 2018 3,956 2 View

How to fix free-standing polymer samples on Si-wafer for Atomic Force Microscopy?

Hello, I would like to perform an AFM measurement to my free-standing, polymer-based composites. I have found some special adhesives for this purpose on the internet, however we do not have any...

26 February 2018 6,841 1 View

How can I prepare AESO as a resin for composite production ?

... in terms of initiator, accelerator, hardener additions and their ratios

16 February 2018 5,454 3 View

Respondents for test-retest reliability?

Hello, How many respondents are necessary for test-retest reliability? In my opinion, with test-retest reliability men analyze the correlation between two different scores therefore respondent...

28 December 2017 5,028 3 View

Secure and Anxious Attachment ?

Hello all, We all know the theoretical basis of attachment theory of Bowlby. In the latest literature, the attachment theory is applied to both romantic relationships and relationships with God...

18 December 2017 5,845 1 View

How can I coat Zn to the glass/quartz substrate?

Zn is not coated to the glass/quartz substrate. I tried by PVD, but it's impossible. What can be the reason behind this and do you have any suggestions?

04 April 2016 3,542 15 View

SEM with AMOS or LISREL?

Hi, For the first time I want to analyze my data with using Structural Equation Modeling. I have read too much theoretical books about SEM. I draw the schematic presentation of my variables...

24 February 2016 2,273 8 View

Does anyone have any idea why I only have low weight size in western blot?

09 June 2015 2,615 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View