I need to perform Immunohistochemistry on brain tissue and hence was planning to perform transcardial perfusion, however, I was not sure if this will impact measurement of ROS and enzymes relevant to oxidative stress. I will be greatful if anyone can help me out in this regard.
Thank you!
Hi Lakshman Puli, I'm not specialist in this analysis in animal. But I did thousand times in plant tissues. If this perfusion cause any damage in tissue I can tell you that you'll find alterations in your parameters, beside on the nature and importance of it nature. My guess for you is try to minimize the stress. For example, the sample that you perform the perfusion frozen it how fast you can. Then, in time to do the analyzes put the sample in buffer.
I hope that I can help you somehow.
Have a nice day.
i have done such experiments and in the beginning we came-over such problem but by the time we came to the conclusion that liver tissue samples shouldnt be delayed for measurement. but in case it is delayed and perfusion occur the tissues should be freezed and then kept in buffer