Dear all
I work with colorectal and many other solid cancer cell lines. I recently come across some contamination in my Ht29 cell lines and which later contaminated other cells too. the cell morphology is unclear with ill-defined cell margins. It might be due to frequent inspections of service personnel to resolve water condensation in our Co2 incubator. As this is my first encounter of contamination, i am not able to identify it (i guess its fungal).anyway i am planning to discard all cultures and revive from stock. with the help of attached pictures can anyone help me to identify the issue please.
thanks in advance
I don't think this is fungal contamination, it is more like mycoplasma. Click here to help you...https://www.creative-bioarray.com/products/mycoplasma-detection-elimination-211.htm
You will want to run a sterilization cycle on that incubator, replace the water tray (if you are using one) with a clean preferably sterile tray and sterile water also. You may also want to consider a Virkon clean. Contamination will probably continue to occur if not.
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