I am using the Promega dual-glo assay and I'm getting replicates which are far apart. I gather this might be a technical issue but just to be sure is there a standard curve I can use in analyzing my data?
This protocl should help.
Good luck!
https://mynotebook.labarchives.com/share/Michael%27s%20Lab%20Notebook/NTY0LjJ8MTQ5MjIvNDM0L1RyZWVOb2RlLzM0NjYwMjI5MDd8MTQzMi4y
Dear Nana,
Luciferase assays need calibration using a standard curve. This must be made with the type of luciferase that is assayed as different luciferases have different specific activities.
The variation between duplicates can be caused in many ways. One of them is inactivation by binding to microplate wells or cuvette walls. You can try our kit https://biothema.com/applications/reporter-gene-assays-and-in-vivo-imaging/.
Best regards,
Arne
Thank you Savita Visal-Shah and Arne Lundin . I will take a look at the links
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