It's my first time working with this virus, I would like some advices!!
Hello everyone, We are trying to differentiate the KOLF-NGN2 hiPSC into neurons - A reference human induced pluripotent stem cell line for lar... . The differentiation is characterized by 2...
09 July 2024 4,002 1 View
Moderation, multicollinearity
30 April 2024 9,574 5 View
Searching for articles or insight on determining the number of vignettes needed for a study using the Factorial Survey Method.
04 March 2024 1,680 3 View
We would like to order a BALB/c strain from Charles Rivers or Jackson laboratories, but apparently there are two such strains, and we are not sure which one is better or if there is any difference...
09 November 2023 5,876 0 View
Arbitrating and Mediating Generative AI Works: The Legal and Ethical Challenges The jurisdiction doesnt really matter i am looking at the possibility of decongesting the docket of the court and...
12 August 2023 6,835 5 View
How can I create a free DOI?
05 August 2023 6,507 1 View
RNA isolation
29 June 2023 5,130 2 View
I used MATLAB code on NRW method to measure the complex permittivity of my nanocomposite samples. The results I got using it were very unexpected. I rechecked my code many times but did not...
15 May 2023 3,955 0 View
We have infected mice with an AAV-EGFP and we were hoping to directly visualize the fluorescent signal in frozen muscle sections without antibody staining. Any suggestions would be greatly...
12 April 2023 4,058 3 View
A sample of this Question need to be answered in a literature review form
27 February 2023 5,613 11 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,178 2 View
I get two different bands from BCMV after PCR
07 July 2024 2,112 1 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
I need this complete article, could anyone help me? follow theme below. Chikungunya virus cell-to-cell transmission is mediated by intercellular extensions in vitro and in vivo.
20 June 2024 3,373 1 View
Infected potato plant was negative for PVY, PVX, PVM, PVS. What can it be?
19 June 2024 6,739 3 View
Hello everyone, I'm currently working on a project where I need to isolate viruses in culture from incubation with PCR-positive serum samples, but I'm facing the challenge of dissociating...
17 June 2024 1,855 1 View