Rita Corrales

5 Questions 2 Answers 0 Followers

Questions related from Rita Corrales

How to apply the RNA purification using Trizol for viruses contained in culture media?

Hello everybody, I have to apply a Trizol protocol for the purification of RNA in viruses contained in culture media; in fact, it is the first time I use these techniques!!!! Our goal is to...

27 January 2023 9,552 3 View

Can I use a microplate reader 800 TS biotek for diagnostic ELISA? that already have a standard factor and I don't need to use any standard curve?

Hello, I bought a Micro-plate reader 800 Ts Biotek, it has a Gene5 3.11 software. However, I want to use it for Diagnostic ELISA and I've already have the standard factor, so I don't need to...

24 June 2022 8,192 16 View

I was wondering if somebody have done before a Titration and PRNT of Yellow Fever Virus?

It's my first time working with this virus, I would like some advices!!

02 November 2021 2,975 0 View

Hi, I was wondering if somebody here have done a western blotting of Leishmania by using the cultive of parasite? has it a different treatment?

I'm working with Leismania panamensis and I got a problem using the pellet for western blotting. I collected a cultive of parasite and washing 3 times with PBS after that I used a DTT and sample...

06 October 2021 9,689 5 View

I got a Nitrotetrazolium Blue chloride bottle instead of MTT and I don't know how to use it for cell viability, Have anyone some ideas?

Examples of protocols for NBT ( Nitrotetrazolium Blue chloride ) in cells viability assay

18 February 2020 8,809 1 View