I want to study ROS1 and RET gene fusion in Lung cancer based on RT-PCR in human FFPE samples (the fusion partner genes of ROS1 (6q22)  are SLC34A2 (4q15), CD74 (5q32) and SDC4 (20q12 and RET are KIF5B and  CCDC6).

I am planning to make fusion specific primers. If their is fusion then bands will be seen in gel otherwise not. But for doing that do i require positive control also.