Varsha Singh

3 Questions 7 Answers 0 Followers

Questions related from Varsha Singh

Can anybody help me how can I use restriction digestion for studying point mutation and complete exon deletion?

I want to study Point mutation and complete exon deletion in gDNA/cDNA. I am new to restriction enzyme field. How should I choose my enzymes for the same. Should I use Blunt end or overhang...

02 February 2017 8,896 8 View

I want to study rearrangement of ROS 1 and RET genes in FFPE sample?

I want to study ROS1 and RET gene fusion in Lung cancer based on RT-PCR in human FFPE samples (the fusion partner genes of ROS1 (6q22) are SLC34A2 (4q15), CD74 (5q32) and SDC4 (20q12 and RET are...

12 December 2016 949 1 View

Can anyone suggest me how can i study rearrange(fusion) genes at gDNA level in lung cancer?

I want to study gene rearrangement(fusion) of ALK, ROS1 and RET genes in lung cancer at gDNA level. What is the best possible way for it? Is it fine to make primers specific to fusion area and...

11 November 2016 1,944 2 View