What should be sample size of traning dataset for applying data mining techniques?

21 December 2020 8,119 3 View

Can I combine data from two separate fluorometeic experiments?

I performed a fluorometric experiment with two different cell lines. I had four conditions per experiment, and four "sub-conditions" per condition, wherein I add three different chemicals (the...

08 November 2020 5,113 3 View

Is it possible to calculate Absorption co-efficient or Absorbance of a dye theoretically?

I am working on a Luminescent Solar Concentrators (LSC) using dyes such as Coumarin 1, Coumarin 153, and Perylene Green Ph-probe 720-5.5. I need to calculate the Mean-free-path (MFP) for the...

18 August 2020 9,804 3 View

Is ACE inhibition linked to COVID-19?

There is increasing chatter that proteins in the ACE2 receptor binding domain are related to COVID-19 entering humans cells and subsequently infecting the host. Is there any evidence of ACE...

19 March 2020 6,635 8 View

In Phospharic Acid plant , Calendria is being used is generally of Graphite tube- shell & tube heat exchanger. Anyone using experience SS316 tubes?

Presently Majority of PA plants are using Graphite tubes in their system oh concentration for heat-exchange. Reliability is little less. We want to know that is ANY PA producing plants is having...

13 March 2020 4,877 3 View

How to model blind bolts in ABAQUS?

I am trying to simulate a beam-column connection using blind bolts. but I am not getting an idea to model blind bolts in Abaqus. please give me some idea of modelling. thanks in advance

05 February 2020 8,916 3 View

Using continuos glucose monitoring system and glycemic index of foods?

Hello All, Can someone please share a protocol/ methodology for testing GI (glycemic index) of a food using CGMS (continuos glucose monitoring system). Is this kind of testing approved by FAO/...

26 August 2019 3,090 2 View

What are the possible ways to increase the internodal distance of in vitro cultures?

I am working on Papaya micropropagation where I am getting plenty of shoots but they are not increasing in height. I have tried diffuse light, GA3 but did not see improvement. Please suggest a...

03 June 2019 812 23 View

Reduction in fasting Insulin levels and associated CVD/T2D risk?

Does anyone have papers relating fasting insulin levels and associated CVD risks?

03 November 2018 4,281 3 View

What is the relationship between Stroke Volume and Diastolic BP?

Does stroke volume and diastolic BP have a positive linear correlation? Given greater pre-load and thus end-diastolic volume is it expected that diastolic BP would be reduced?

14 December 2017 9,316 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View