How to calculate number of amine molecules attached/ immobilization density on a surface (surface area)? Is fluorescence labeling or assay needed?

Name of assay, fluroscence molecule should be used for knowing the number of amine molecules immobilized.

07 August 2018 9,331 5 View

How to prevent quenching in a graphene layer coated with amine molecules? I want to measure number of amine molecules. What Surfactant to be used?

What kind of surfactant(to prevent quenching) and assays to be used? To know number of amine molecules on a graphene layer.

07 August 2018 2,530 0 View

Is there any review paper or articles based on sensors for electric vehicles? Papers related to sensors (not on battries of EV's)?

I don't need papers on batteries . I need papers on sensors in electric vehicles may be analog or digital both. Also, papers on mems based sensors for EV's.

06 July 2018 8,511 1 View

Is accelerometer and gyroscope need in electric vehicles/ Battery electric vehicle? . What kind of mems based sensors are needed in electric vehicles?

mems, accelerometer , gyroscope, mems sensors in electric vehicles.

06 July 2018 1,300 11 View

How to calculate atomic percentage of various groups in Graphene oxide from xps data? If atomic percent of C1s 60% and O1s 30%?

atomic percentage of various groups in Graphene oxide needed.

05 June 2018 2,824 4 View

Can plastic deformation in polymer increase crosslinking density of the polymer?

Cross linking density increase due to plastic deformation

04 May 2018 7,902 13 View

Can anyone provide me a related paper on effect of plastic strain (i.e. permanent deformation) of polymers on the cross-linking network density?

effects of plastic deformation on cross linking density of polymer

04 May 2018 3,543 0 View

Query on stifness and strain energy density with thickness?

For a linear elastic material what relation does the strain energy density function bears with the stifness of the material?Further I want to know how strain energy density function relates with...

07 August 2017 496 1 View

We recently bought Dengue NS1 antigen and monoclonal antibody at IIT KANPUR.I need to know the thawing temperature and process NS1 antigen antibody?

01 February 2017 1,106 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View