I want to show the binding between Pol-l-lysine and glucose oxidase or any other similar type enzyme using two fluorescent dyes. Dye for tagging?

More Aviru Kumar Basu's questions See All

How to calculate number of amine molecules attached/ immobilization density on a surface (surface area)? Is fluorescence labeling or assay needed?

Name of assay, fluroscence molecule should be used for knowing the number of amine molecules immobilized.

07 August 2018 9,407 5 View

How to prevent quenching in a graphene layer coated with amine molecules? I want to measure number of amine molecules. What Surfactant to be used?

What kind of surfactant(to prevent quenching) and assays to be used? To know number of amine molecules on a graphene layer.

07 August 2018 2,605 0 View

Is there any review paper or articles based on sensors for electric vehicles? Papers related to sensors (not on battries of EV's)?

I don't need papers on batteries . I need papers on sensors in electric vehicles may be analog or digital both. Also, papers on mems based sensors for EV's.

06 July 2018 8,630 1 View

Is accelerometer and gyroscope need in electric vehicles/ Battery electric vehicle? . What kind of mems based sensors are needed in electric vehicles?

mems, accelerometer , gyroscope, mems sensors in electric vehicles.

06 July 2018 1,391 11 View

How to calculate atomic percentage of various groups in Graphene oxide from xps data? If atomic percent of C1s 60% and O1s 30%?

atomic percentage of various groups in Graphene oxide needed.

05 June 2018 2,936 4 View

Can plastic deformation in polymer increase crosslinking density of the polymer?

Cross linking density increase due to plastic deformation

04 May 2018 8,006 13 View

Can anyone provide me a related paper on effect of plastic strain (i.e. permanent deformation) of polymers on the cross-linking network density?

effects of plastic deformation on cross linking density of polymer

04 May 2018 3,632 0 View

Query on stifness and strain energy density with thickness?

For a linear elastic material what relation does the strain energy density function bears with the stifness of the material?Further I want to know how strain energy density function relates with...

07 August 2017 635 1 View

We recently bought Dengue NS1 antigen and monoclonal antibody at IIT KANPUR.I need to know the thawing temperature and process NS1 antigen antibody?

We recently bought Dengue NS1 antigen and monoclonal antibody at IIT KANPUR.I need to know the thawing temperature and how to thaw the NS1 antigen and antibody.One of my labmate thawed at 35...

01 February 2017 1,190 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View