I want to show the binding between Pol-l-lysine and glucose oxidase or any other similar type enzyme using two fluorescent dyes. Dye for tagging?

FITC-TRITC protocol when both used together? any other florescent dye for tagging?

10 November 2018 790 3 View

How to calculate number of amine molecules attached/ immobilization density on a surface (surface area)? Is fluorescence labeling or assay needed?

Name of assay, fluroscence molecule should be used for knowing the number of amine molecules immobilized.

07 August 2018 9,331 5 View

Is there any review paper or articles based on sensors for electric vehicles? Papers related to sensors (not on battries of EV's)?

I don't need papers on batteries . I need papers on sensors in electric vehicles may be analog or digital both. Also, papers on mems based sensors for EV's.

06 July 2018 8,511 1 View

Is accelerometer and gyroscope need in electric vehicles/ Battery electric vehicle? . What kind of mems based sensors are needed in electric vehicles?

mems, accelerometer , gyroscope, mems sensors in electric vehicles.

06 July 2018 1,300 11 View

How to calculate atomic percentage of various groups in Graphene oxide from xps data? If atomic percent of C1s 60% and O1s 30%?

atomic percentage of various groups in Graphene oxide needed.

05 June 2018 2,824 4 View

Can plastic deformation in polymer increase crosslinking density of the polymer?

Cross linking density increase due to plastic deformation

04 May 2018 7,902 13 View

Can anyone provide me a related paper on effect of plastic strain (i.e. permanent deformation) of polymers on the cross-linking network density?

effects of plastic deformation on cross linking density of polymer

04 May 2018 3,543 0 View

Query on stifness and strain energy density with thickness?

For a linear elastic material what relation does the strain energy density function bears with the stifness of the material?Further I want to know how strain energy density function relates with...

07 August 2017 496 1 View

We recently bought Dengue NS1 antigen and monoclonal antibody at IIT KANPUR.I need to know the thawing temperature and process NS1 antigen antibody?

01 February 2017 1,106 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View