I want to perform MTT assay on fish cell. Have anyone done that before?

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Daniel William Hutchins

Due to the lower temperature (usually run in a cell culture incubator at 35-36 C) you may need to increase the time you incubate the cells with MTT.

Nazanin Mohebali

Dear Daniel,

thanks for your answer. I actually tried that but after 20 hours, I read the plate with no toxicity by OD: 0.1

What do you think could be the problem?