Nazanin Mohebali

5 Questions 10 Answers 0 Followers

Questions related from Nazanin Mohebali

How to optimize periplasmic protein expression in E.coli (BL21)?

I want to optimize protein purification. My protein is expressed in periplasmic space of E.coli. I particularly want to know what is the routine protocol for these kind of proteins. Is it 1mM...

17 July 2017 5,257 7 View

I want to perform MTT assay on fish cell. Have anyone done that before?

I did the experiment as described in protocols. but since my fish cells only sty alive in 18 degrees, I did MTT assay in this temperature. The only problem was that I read very low optical density...

18 February 2017 7,028 2 View

Is reduced FBS concentration involved in attachment of cells?

I am working on cell line establishment from fish. I am using 20% FBS but recently I have read somewhere that FBS 2% is used for attachment of cells in the first day and then 10% FBS is used for...

10 December 2016 5,578 3 View

What can I add to the fish cell culture medium to improve cells situation?

I'm using Leibovitz L-15 medium complemented with 20% FBS, 2mM L-Glutamine, 1mM sodium pyruvate, Does adding 2-ME (mercaptoethanol) help freshen the cells?

18 June 2016 6,700 7 View

How much amyloid beta is needed to make 1 mouse model for Alzheimer's?

I'm currently working on a project in which I need an Alzheimer's mouse model. I need a reliable protocol to make the mouse model.

07 April 2014 8,006 5 View