Nazanin Mohebali

3 Questions 9 Answers 0 Followers

Questions related from Nazanin Mohebali

How to optimize periplasmic protein expression in E.coli (BL21)?

I want to optimize protein purification. My protein is expressed in periplasmic space of E.coli. I particularly want to know what is the routine protocol for these kind of proteins. Is it 1mM...

17 July 2017 5,174 7 View

What can I add to the fish cell culture medium to improve cells situation?

I'm using Leibovitz L-15 medium complemented with 20% FBS, 2mM L-Glutamine, 1mM sodium pyruvate, Does adding 2-ME (mercaptoethanol) help freshen the cells?

18 June 2016 6,644 7 View

How much amyloid beta is needed to make 1 mouse model for Alzheimer's?

I'm currently working on a project in which I need an Alzheimer's mouse model. I need a reliable protocol to make the mouse model.

07 April 2014 7,974 5 View