I want to estimate nitric oxide released by the endothelium of mouse aorta. I plan on explanting the aorta, cutting 5mm segments and maintaining them in Krebs ringers for a short while for the experiment. Can i use the buffer the aorta segments were in to measure nitric oxide evolved?
You might consider trying the Griess assay, which measures nitrite as an indicator of NO release. It's pretty straightforward and Invitrogen sells a kit for it.
If you can do fluorescent imaging on your segments, detecting NO using DAF dyes (Molecular probes) might also be possible.
Harvard Apparatus makes electrochemical probes for NO, but I don't have any experience using those.
Good luck!
I have used Cayman Nitrate/nirite kit (Cat No.: 780051) and it works excellently for even lower amounts of nitrate or nitrite levels.
Reffer to page 10 of - https://www.caymanchem.com/pdfs/780051.pdf
You can use DAF to determine NO release from endothelium.
Other measurement inclure DAN (diaminonapyhalene)- fluorescent method of estimating nitrite/nitrate. this is more sensitive than griess. Usually usd to detect NO from endothelium and neuronal celllines (as the NO produced are relatively low)
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