you need to add restriction site with extra bases to the 5'end of your primers,here link for this protocol:

https://www.addgene.org/protocols/pcr-cloning/

you need to add restriction site with extra bases to the 5'end of your primers,here link for this protocol:

https://www.addgene.org/protocols/pcr-cloning/

Hello,

You will need to add the restriction site and 5 extra bases as a tail in your PCR primer (the 5 extra bases allows efficient digestion of the linear PCR amplicon). Check this link for different enzyme requirement for additional bp (https://international.neb.com/tools-and-resources/usage-guidelines/cleavage-close-to-the-end-of-dna-fragments). I have created a little image that details how to construct this more visually along with some notes on how to create the primers.

Best wishes,

Hanna

I think you have got your answer.

Thank you so very much. i am gonna try see if it works.