What's the analytic plan for a longitudinal design (two waves) with gender as a moderator in the relationship between an iv and a dv ?

hello, every researcher, If I want to study the relationship between an iv and a dv, and the moderation effect of gender, will the analytic plan shown below be appropriate? 1. MANOVA and Post...

26 November 2018 5,402 3 View

What you can do for a thin and good tem-sample?

Does anyone do the reaserch about graphitic carbon materials,if you want to make a beatiful TEM image ,what you can do for a thin and good tem-sample?I want to make my sample thiner and like one...

07 September 2015 4,116 4 View

How can I make activited carbon or amphours carbon become graphite?

My sample only partially graphitized, I hope that it will fully graphitized, or some high degree of graphitization.(the Raman specturm of my sample after High-temperature annealing)

03 April 2015 4,401 4 View

How do I do exfoliation graphite in NMP with a low power sonic bath ?

27 March 2015 3,616 3 View

What intermediate or adhesive layer can I use for depositing silver on glass by electron beam evaporation?

15 September 2014 7,475 5 View

How is the reproducibility of experiments in electrochemical impedance spectroscopy (EIS)?

It puzzles me when I try to repeat EIS experiments. The same experimental conditions are used, unless the bare electrode is cleaned between each run. Different EIS data is obtained in terms of the...

02 June 2014 9,177 7 View

Can anyone give an explanation of the causes of a strange high frequency response in electrochemical impedance spectroscopy?

I use bare Au or Pt electrode as the working electrode. Ag/AgCl and Pt wire are the reference and counter electrode, respectively. 1 M KNO3 is chosen as the electrolyte. Open circuit potential is...

27 May 2014 140 14 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View