What is U matrix for Hosking & Wallis discordancy test based on L-comoments?

Hello everyone I would compute Hosking & Wallis discordancy test based on L-comoments for multivariate Regional Frequency Analysis. Please help me by answer to these questions. 1- Is that...

28 November 2020 8,152 2 View

Is geographical neighborhood important for regional frequency analysis?

Hello all. I have 95 stations with different climatic variables. I categorized those stations into 5 homogeneous group, but for some groups there is no geographical neighborhood. Can i apply...

11 October 2020 6,190 3 View

Does anybody know some prominent books in Cognitive Linguistics?

I would like to carry out my research and need to know more about the cognitive linguistics and psycholinguistics.

16 April 2020 9,368 8 View

What is the best way to merge two different neural networks which are trained for the same task but on different datasets?

I want to train two deep neural networks on two different data sets. The aim is same in both ( predicting cancer relapse) but data sets contain different type of information. I am looking for the...

02 September 2018 9,480 8 View

Does anyone know any reference that has some decision theory examples in building construction or renovation?

I am looking for a book or another reference which has different examples of decision theory in building construction. for example, some examples that show different actions and their outcomes...

30 July 2018 2,115 1 View

How to find lattice constant with calculation of lattice strain?

08 May 2017 9,517 2 View

What does it mean if the negative control shows bands on agarose gel after PCR?

09 October 2014 908 10 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View