i want to know about touch down PCR more
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
In the protocol that I have, I have to break the bacterial samples with an amplitude of 100 in 3 cycles of 3 minutes. But the power of the device is not mentioned. Can I calculate it?
30 August 2023 4,198 0 View
I wish to protect the protein from proteinase enzymes when using (yeast) to purify human papillomavirus protein, Which enzyme inhibitor is alternative to PMSF? which to be stable in high...
24 July 2023 7,268 0 View
I am going to do a study using an eye tracker. I am wondering if I can recruit participants from other countries?
19 June 2023 1,969 4 View
I want to calculate the binding energy between ligand and receptor. And I'm so confused. please instruct me.
08 April 2023 6,086 0 View
Is there any software that shows the interactions between two monomers? and shows the residues which involve in dimer formation?
10 March 2023 634 1 View
To determine whether changes in cell cycle are related to DNA damaged, we investigated the effect of our drug on cell cycle distribution by flow cytometric analysis. also the tested drug,...
18 August 2021 4,053 1 View
I calculated the combination index with CompuSyn software, Among 8 combination data points, 5 of them are on the antagonism side (CI>1), the other 2 points are nearly additive and one of them is...
20 July 2021 752 7 View
Hello everyone I would compute Hosking & Wallis discordancy test based on L-comoments for multivariate Regional Frequency Analysis. Please help me by answer to these questions. 1- Is that...
28 November 2020 8,409 2 View
I have started working on viscoelasticity (with very limited background) and our preferable model in this study is Giesekus. We have Capillary Rheometer and Rotational Viscometer accessible in the...
10 November 2020 1,707 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...
16 July 2024 118 2 View
