I wish to protect the protein from proteinase enzymes when using (yeast) to purify human papillomavirus protein, Which enzyme inhibitor is alternative to PMSF? which to be stable in high temperature, 25 to 34 °C?
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
In the protocol that I have, I have to break the bacterial samples with an amplitude of 100 in 3 cycles of 3 minutes. But the power of the device is not mentioned. Can I calculate it?
30 August 2023 4,198 0 View
I am going to do a study using an eye tracker. I am wondering if I can recruit participants from other countries?
19 June 2023 1,969 4 View
I want to calculate the binding energy between ligand and receptor. And I'm so confused. please instruct me.
08 April 2023 6,086 0 View
Is there any software that shows the interactions between two monomers? and shows the residues which involve in dimer formation?
10 March 2023 634 1 View
To determine whether changes in cell cycle are related to DNA damaged, we investigated the effect of our drug on cell cycle distribution by flow cytometric analysis. also the tested drug,...
18 August 2021 4,053 1 View
I calculated the combination index with CompuSyn software, Among 8 combination data points, 5 of them are on the antagonism side (CI>1), the other 2 points are nearly additive and one of them is...
20 July 2021 752 7 View
Hello everyone I would compute Hosking & Wallis discordancy test based on L-comoments for multivariate Regional Frequency Analysis. Please help me by answer to these questions. 1- Is that...
28 November 2020 8,409 2 View
I have started working on viscoelasticity (with very limited background) and our preferable model in this study is Giesekus. We have Capillary Rheometer and Rotational Viscometer accessible in the...
10 November 2020 1,707 1 View
Hello all. I have 95 stations with different climatic variables. I categorized those stations into 5 homogeneous group, but for some groups there is no geographical neighborhood. Can i apply...
11 October 2020 6,393 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...
28 July 2024 6,553 1 View
AGILENT HPLC 1100 SERIES operation assistance needed.
25 July 2024 10,045 2 View
I have designed magnetic material with different biasing conditions in HFSS. Now I want to give an RF AC signal and do a transient simulation in HFSS. Is it possible to do in HFSS? Please help me...
24 July 2024 9,319 5 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View