I want to isolate reticulocytes from the red blood cells?

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TC28a2 cells detaching from 12-well plate after staurosporin treatment?

Hello everyone, I am facing a frustrating issue with the TC28a2 cell line. After treatment with staurosporine at concentrations of 25nM, 50nM, and 100nM, the cells begin detaching during the...

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How dock a protein with CSO residue?

In working with a protein that possesses a sulfenic acid cysteine in the active site (Cys-SOH/CSO) I`m trying to perform a molecular docking using Auto dock vina. But, in the pdbqt file, the CSO...

14 September 2022 4,432 1 View

What is the best device to identify gold and estimate the volume of a primary gold ore?

In the market, there are many devices that offer varying features to hunt natural gold. I am very suspicious if these devices really delivery what they say. The prices vary considerably and some...

16 November 2020 3,177 4 View

How to overlay and align different scale raster?

I need to overlay two rasters and the pixels of they must be in the same position to each other. I need this to perform a ecological niche model using a package in R. For this end all images must...

20 August 2020 9,712 4 View

How to do a social network analysis based on forum interactions?

I would like to do a social network analysis based on the forum interactions messages, but I don't know how to build the database for it. Can you help me? Thank you Rogério Costa

02 August 2020 9,047 4 View

How to choose good K-point sampling for structure optimization in DFT?

Geometric optimization of a structure with DFT is always the most time demanding step in my calculations. A simple point defect in a 2x2x2 supercell and there goes a week doing relax calculation....

12 July 2020 3,549 10 View

How to infer Causality in a observational data?

My Machine Learning model show in different ways, high correlation between parents' education and student's outcomes. I 'm trying to identify a causal relationship in a separate model since the...

11 June 2020 4,389 7 View

What is the origin of chlorine for sea water?

Sodium and chlorine are present in aqueous fluid inclusions in lacustrine continental (non-marine) rocks. What is the origin of this two chemical components? What is the primary source of them for...

07 May 2020 3,457 6 View

Plasminogen activator inhibitor type 1 (PAI-1), Acibind PAI-1 Kit Technic problems?

If someone can help use for understand what are we doing wrong. Acibind PAI-1 Kit Results, we found that although the Controls and Calibrators performed well according to specifications, several...

21 August 2019 7,904 3 View

Can anyone give suggestion were i can find a platform on ANOVA variance analysis? any website or Research articles?

Dear can anyone give suggestion were i can find a platform on ANOVA variance analysis? any website or Research articles? (analysis of variance, a statistical method in which the variation in a...

31 January 2019 4,119 6 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

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PBMC infection with virus?

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01 August 2024 4,403 4 View

How can I get my Granzyme B flow cytometry stain to be consistent?

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Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

José Ramón Vielma

Enrichment of reticulocytes from whole blood using aqueous

multiphase systems of polymers

The Isolation of Reticulocyte-Free Human Red Blood Cells

Article in Experimental Biology and Medicine · December 2007

Improved Isolation of Normal Human Reticulocytes Via Exploitation of Chloride- Dependent Potassium Transport

By Martin P. Sorette, Kathleen Shiffer, and Margaret R. Clark