Can I use soft vacuum filtration to harvest Ath seedlings from liquid media?

Hello, I'm growing different accessions of Arabidopsis thaliana and so far we have been harvesting them by pouring the media over a simple sieve, then putting them on paper towels for a minute...

18 February 2020 7,944 3 View

How do you know what plants have been examined for natural products and their activity?

I am an NMR scientist looking to do some side research in Pharmacognosy/Natural Products in my side time. I'm looking to see what plants have been examined/screened but have not found a central...

29 July 2018 8,632 3 View

Is there any notable research, or specific examples, which look at the interference (good or bad) of democratic politics in public water management?

I am well aware that all infrastructure and water management planning functions are to some extent political in nature. Apologies for a very broad question, I am interested in other researchers...

22 February 2016 2,148 4 View

What is the present Delta T/T of the CMB anisotropy from WMAP 9 or PLANCK?

Cannot find this value in either WMAP 9 or PLANCK data

08 April 2015 5,515 9 View

What is the boiling point of chitosan and lignin?

I am thinking of reduction the chitosan (or) lignin modified graphene oxide in the furnace, so that I need to know their boiling point. Thanks.

30 October 2014 5,248 4 View

Anyone out there doing "big data" work in the big ocean?

I am interested in any big data projects that are focused on ocean data and information. "Big data" is loosely defined as data so complex that they can not be processed or managed using...

12 March 2013 9,974 5 View

Making monocyte lysates.

I'm interested in making a monocyte lysate as a positive control for a Western Blot. The protein of interest is TLR2. What cell count would I need to make a lysate with approximately 1mg/mL...

05 February 2013 4,188 1 View

Has anyone tried the Thiobarbituric acid reactive substances (TBARS) assay for LDL and had any success?

I've found a protocol online but so far am not having much luck with it! I was wondering if anyone else was having similar difficulties or is it just me?!

08 January 2013 7,800 6 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

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22 February 2021 9,932 11 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View