We have isolated primary human bronchial epithelial cells and frozen them down. After thawing and culturing them, and having identification done, it appears that we have contamination from the Klebsiella genus. Has anyone had this happen? Would this be something from the donor, or cross-contamination in the isolation process?
Karen A. Darbinyan
Hello, human bronchial epithelial cells itself can contain(probability about 30%) contamination as well as it can be result of cross-contamination (probability about 70%) during isolation process. If it's not forbidden for future use you can use the antibiotic solution some representative of Cephalosporins during isolation process.
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