Check that Repli-G MDA reaction worked before sending product for sequencing?

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Prevent evaporation in hypoxic incubator?

I have noticed massive media evaporation (>50% volume) from culture dishes after 72-96hr inside a Memmert ICO50 hypoxic incubator (Temp: 37C, humidity: 86%, CO2: 5%, O2: 1%). I've confirmed the...

29 April 2024 9,872 1 View

How does the stability of routine biochemical analytes in whole blood and plasma from lithium heparin gel tubes change during a 6-hour storage period?

The inquiry relates to a study conducted by Alkouri, Rana, et al. in which they investigated the stability of various biochemical analytes in whole blood and plasma samples stored for different...

15 April 2024 3,962 0 View

What are the preparations needed to be followed by the patient during the collection of their urine & how can microbiologist ensure that the patient?

The question is based on the article, “Tenets of Specimen Management in Diagnostic Microbiology” by Rajeshwar and Pathak. It aims to know how the microbiologist can help the patients do their part...

05 February 2024 9,935 3 View

How can I interpret this ITC result?

I am using isothermal titration calorimetry to measure the binding affinity and stoichiometry between a small molecular weight poly(amino acid) (3500 - 4500 Da) (SYRINGE) and high molecular weight...

02 August 2023 6,564 8 View

Can any one help me interpret the results of a restriction digest of a plasmid DNA the vector was pET22b(+) and the enzymes was Nde1 and EcoRI?

the cloning , transfornation and plasmid prep was done in the a series of lab sessions too . The pet22b(+) vector was combine with the ALDH gene . The digestion is done on the eluted DNA from the...

26 October 2022 2,660 1 View

Using Gen5 Cytation for Micro BCA Assay - How is a protocol set up with unknown dilutions?

I am trying to using the Gen5 Cytation by BioTek for protein quantification using a micro BCA Assay kit. I downloaded a BCA Assay Experiment (.xpt) File, and tried to use it but ran into some...

21 June 2022 650 3 View

I want to elute using a pH gradient for a protein bound to Ni-NTA resin. What are the optimal solutions to create the gradient?

I am trying to purify a His bound protein off of a Ni-NTA resin and cannot find a protocol that says the chemical make up of the solutions used to create the gradient.

16 March 2022 412 0 View

Can I use soft vacuum filtration to harvest Ath seedlings from liquid media?

Hello, I'm growing different accessions of Arabidopsis thaliana and so far we have been harvesting them by pouring the media over a simple sieve, then putting them on paper towels for a minute...

18 February 2020 8,139 3 View

Are you considering genetic genealogy sources for YSNP and YSTR. GEDMATCH is being used daily now by US law enforcement but not YSNP & YSTR data?

With 10M testers with atDNA and public access via GEDMATCH, dozens of law enforcement agencies in the US are using this source with amazing results for cold cases and unidentified remains. YDNA...

23 January 2019 1,280 5 View

How to address and/or avoid Klebsiella contamination of primary human bronchial epithelial cells?

We have isolated primary human bronchial epithelial cells and frozen them down. After thawing and culturing them, and having identification done, it appears that we have contamination from the...

06 December 2018 762 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

Help. How can I fix my T7 reaction problems?

I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...

16 July 2024 4,604 0 View

Qiagen circulating nucleic acid kit poor yield?

Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...

14 July 2024 7,475 1 View

Are DNA samples with 260/280 ratio greater than 2.1 good for sequencing?

I have DNA samples (extracted by using Qiagen kiit), the 260/280 ratio is consistence 2.1.. for most of the samples. Can I proceed for the whole genome sequencing with these values? NGS.

07 July 2024 3,751 4 View

How can I improve purity of DNA extracted from cyanobacteria using Dynabeads?

I am trying to extract DNA from the cyanobacteria Microcystis aeruginosa using the Dynabeads DNA DIRECT Universal kit for the purpose of on-site detection. Yield is acceptable at ~100-500 ng/uL,...

01 July 2024 10,003 1 View

Does Trizol LS yield more and better RNA than normal Trizol?

Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...

18 June 2024 6,854 3 View

How to pellet adipocyte spheroids?

I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....

27 May 2024 1,303 1 View

DNA purification from ATCC 25922 E.Coli strain with QIAGEN kits?

Hi everyone, We've got a plasmid we want to purify from E.Coli strain ATCC 25922. We usually use QIAGEN plasmid purification kits but we've never purified DNA from this particular strain. Would...

20 May 2024 3,803 1 View