I want to do a simulation of a tRNA containing GDP and/or GMP , GTP as one of its bases. How do I load this structure in AMBER11 and/or NAMD2.9 ? Please suggest.
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Dear Sir, I hope this message finds you well and in good health. I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master....
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Literature on the above subject will be welcomed
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I am planning to design guideRNA for multiple targets to express as a polycistronic-tRNA-gRNA expression. I would like to know whether we need to include any linker between the tRNA and guideRNA...
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I am just start learning cell-free protein expression, i want to construct an E. coli extract based system,and found Roche tRNA from E.coli MRE600(10109541001) has discontinued. So i want to...
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I run E coli total RNA on 10% TBE-Urea PAGE denaturing gel, and just wanted to know what RNA species these bands exactly are. I used an RNA ladder contains ssRNA of 1000, 800, 600, 400, 300, 200...
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Hi, I need to amplify a tRNA fragment in a normal PCR, The primers I designed suppose to give me a product of 40 base pairs. I want to transform it into pGEM and send it to Sanger sequencing . My...
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tRNA derived fragments are a novel regulatory ncRNAs with distinct biological functions in cancers and stress-induced diseases. Our research group has worked on tRFs for a while. The major method...
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I want to isolate tRNA from adherent cells (DLD1) which will be used for further screening assays. is there any kit available or any protocol that I need to follow? Its a bit urgent. Thanks in...
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I designed a aptasensor for tRF detection. I need to know what could be the usual practice for tRF concentration range to have a convincing application for plasma tRF detection? I need to...
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