I designed a aptasensor for tRF detection. I need to know what could be the usual practice for tRF concentration range to have a convincing application for plasma tRF detection? I need to understand it in ng/mL unit. I have the understanding that the tRF concentration would be in the range of 0.01-10 ng/mL for plasma, 50 ng/mL for urine, and 0.1-10 ng/mL for cerebrospinal fluid (CSF) samples.
I want to double check with someone who worked in this field and has some experience.
Molecular biology is, unfortunately, not my strong suit.
Thanks.