If the magnetic direction of any two black holes is made equal. So the capacity of attraction decreases to zero?

If the magnetic direction of any two black holes is made equal. So the capacity of attraction decreases to zero? If it becomes zero. So we can make the ability to magnetize / attract other...

22 December 2020 3,062 4 View

Orthogonal array response vectors in poor scattering mmwave MIMO system?

Can anybody help in understanding how in a MIMO system with large (ideally infinite) number of transmit/receive antennas and poor scattering (e.g at mmWave), the transmit/receive array response...

17 December 2020 6,903 4 View

How to change material in ANSYS & perform brittle deformation ?

I am currently working on a model for fracture generation due to pressure applied in ANSYS. When I am working with structural steel material it is showing only folding not any fracture initiation...

16 December 2020 3,826 3 View

Which is the best Instrument for obtaining the geophysical properties of sub-soil?

For performing the real scenario ground seismic simulation, we require certain input parameters like Shear wave variation with depth or Vs (30), density of the soil layers etc. Following...

29 November 2020 9,714 4 View

Purity on nanodrop for the plasmid isolated?

I isolated a plasmid (image attached along with), however the 260/230 ratio was less than desired i.e. 1.29 as compared to the ideal 2.0-2.2. Would it influence the digestion reaction?

09 November 2020 7,666 2 View

How to design the 3D crystal structure of Antibody for protein antigen. Any relevant software/module?

I have a protein (antigen) of size ~ 400 amino acids, for that i need to design antibody 3D PDB file for molecular modelling studies and mechanistic binding analysis to elucidate the molecular...

16 October 2020 4,812 2 View

How to convert artificial neural network (ANN) regression model into Deep learning model?

A am new to machine learning. I have developed an ANN model with 1 hidden layer, to predict sale forecast. As an assignment I have to prepare Deep Learning model for the same task. Deep model has...

21 May 2020 1,137 4 View

Loop for summation in MATLAB ?

for different values of X, what will be the loop or tool in MATLAB for the below problem summation (U(n-L)(X)*(K(L)-K(L+1)), 1, n-1), where U is the function of X for different time (n-L), and...

18 April 2020 7,047 11 View

What is the best buffer for plants to avoid osmotic shock?

Hi! I am doing an experiment where I need to spray a chemical on plant. I will be using autoclaved milliQ water as a base to dissolve small quantities of that chemical. I was wondering how can we...

09 April 2020 5,808 2 View

Can anyone suggest fast publication in SCI or SCIE indexed journal?

I have a requirement to publish a research paper in Science Citation Index (SCI) or Science Citation Index Expanded (SCIE) indexed journal only in the field of Engineering, Manufacturing,...

27 March 2020 6,625 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View