How to check immunogenicity of therapeutic peptides made of unnatural amino acids?

How to check immunogenicity of therapeutic peptides made of unnatural amino acids?

03 April 2019 7,312 1 View

Has anybody expressed MET receptor using cell free protein expression? I need the reference?

Cell free protein expression

11 December 2017 8,293 0 View

Which dipeptide segment plasmin can cut?

We are working with this sequence Leu-Gly-Gly-Ala-Lys-Gln-Ala-Gly-Asp-Val. If we treat with plasmin, which two ions it will generate. Can Plasmin digest any amino acid next to isopeptide bond.

04 May 2017 5,007 3 View

For purifying Bacteriorhodopsin 100 mM PBS buffer should have pH 7.5 or 5.5 and what should be OG conc (w/v%) for solubilization and purification?

This question is with regards to membrane proteins.

08 September 2015 9,117 0 View

How do we improve our TEM imaging for our lipopeptide sample with uranyl acetate staining?

06 July 2015 1,454 4 View

What is the speciality of Opti-MEM media? Can I use DMEM with 5% FBS instead of Opti-MEM media? Why do people use Opti-MEM media for siRNA biology?

I am doing FACS and Immunofluorescence to check transfection of designed transfection reagents.

02 March 2014 596 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View