How to check immunogenicity of therapeutic peptides made of unnatural amino acids?

How to check immunogenicity of therapeutic peptides made of unnatural amino acids?

03 April 2019 7,312 1 View

I need to select membrane model for cancer and metastatic breast cancer for computational studies. I request for your advice.

I need to select membrane model for cancer and metastatic breast cancer for computational studies. I request for your advice.

06 July 2017 1,173 0 View

Which dipeptide segment plasmin can cut?

We are working with this sequence Leu-Gly-Gly-Ala-Lys-Gln-Ala-Gly-Asp-Val. If we treat with plasmin, which two ions it will generate. Can Plasmin digest any amino acid next to isopeptide bond.

04 May 2017 5,007 3 View

For purifying Bacteriorhodopsin 100 mM PBS buffer should have pH 7.5 or 5.5 and what should be OG conc (w/v%) for solubilization and purification?

This question is with regards to membrane proteins.

08 September 2015 9,117 0 View

How do we improve our TEM imaging for our lipopeptide sample with uranyl acetate staining?

06 July 2015 1,454 4 View

What is the speciality of Opti-MEM media? Can I use DMEM with 5% FBS instead of Opti-MEM media? Why do people use Opti-MEM media for siRNA biology?

I am doing FACS and Immunofluorescence to check transfection of designed transfection reagents.

02 March 2014 596 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View