Rituparna Sinha Roy

7 Questions 1 Answers 0 Followers

Questions related from Rituparna Sinha Roy

How to check immunogenicity of therapeutic peptides made of unnatural amino acids?

04 April 2019 7,402 1 View

Has anybody expressed MET receptor using cell free protein expression? I need the reference?

Cell free protein expression

12 December 2017 8,379 0 View

I need to select membrane model for cancer and metastatic breast cancer for computational studies. I request for your advice.

07 July 2017 1,246 0 View

Which dipeptide segment plasmin can cut?

We are working with this sequence Leu-Gly-Gly-Ala-Lys-Gln-Ala-Gly-Asp-Val. If we treat with plasmin, which two ions it will generate. Can Plasmin digest any amino acid next to isopeptide bond.

05 May 2017 5,075 3 View

For purifying Bacteriorhodopsin 100 mM PBS buffer should have pH 7.5 or 5.5 and what should be OG conc (w/v%) for solubilization and purification?

This question is with regards to membrane proteins.

09 September 2015 9,181 0 View

How do we improve our TEM imaging for our lipopeptide sample with uranyl acetate staining?

Electron Microscopy expert We have spotted our lipopeptide sample on glow discharged carbon coated copper grid (1 min) and used freshly prepared 2 % uranyl acetate (2 min). But TEM images are...

07 July 2015 1,603 4 View

What is the speciality of Opti-MEM media? Can I use DMEM with 5% FBS instead of Opti-MEM media? Why do people use Opti-MEM media for siRNA biology?

I am doing FACS and Immunofluorescence to check transfection of designed transfection reagents.

03 March 2014 725 4 View