This question is with regards to membrane proteins.
How to check immunogenicity of therapeutic peptides made of unnatural amino acids?
03 April 2019 7,429 1 View
Cell free protein expression
11 December 2017 8,402 0 View
I need to select membrane model for cancer and metastatic breast cancer for computational studies. I request for your advice.
06 July 2017 1,272 0 View
We are working with this sequence Leu-Gly-Gly-Ala-Lys-Gln-Ala-Gly-Asp-Val. If we treat with plasmin, which two ions it will generate. Can Plasmin digest any amino acid next to isopeptide bond.
04 May 2017 5,097 3 View
Electron Microscopy expert We have spotted our lipopeptide sample on glow discharged carbon coated copper grid (1 min) and used freshly prepared 2 % uranyl acetate (2 min). But TEM images are...
06 July 2015 1,645 4 View
I am doing FACS and Immunofluorescence to check transfection of designed transfection reagents.
02 March 2014 788 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
